A program of Sherbourne Health, Rainbow Health Ontario creates opportunities for the healthcare system to better serve LGBT2SQ communities. RHO offers training for healthcare providers across the province to feel more clinically and culturally competent in caring for their LGBT2SQ service users. RHO also supports system change by producing evidence-based print and web resources, contributing an LGBT2SQ health perspective to public policy processes, acting as a research catalyst and hosting Canada’s largest LGBT2SQ health conference. RHO is primarily funded under the Indigenous, French Language & Priority Populations Branch, Strategic Policy, Planning & French Language Services Division of Ontario’s Ministry of Health. RHO also receives community and donor support.