In the Stress in Action project multidisciplinary scientists from six Dutch Universities collaborate around the theme ‘Stress in daily life’. Divided into three Research Themes and three Support Cores, the Stress in Action consortium will validate daily-life stress assessments, examine which contextual factors contribute to the experience of daily life stress, and examine how daily life stress leads to the development of both mental and cardiometabolic diseases. The project is funded through the Gravitation programme of the Dutch Research Council (NWO). Stress in Action capitalizes on the fast advances in technology and big data analytics to move stress research from the lab to daily life. The consortium enables synergistic collaborations to discover 1) how responses to daily life stress arise from the temporal, dynamic interplay between context and person-specific factors, 2) how daily life stress can be reliably measured in a specific individual in real-time, and 3) how and when potential beneficial stress-response mechanisms turn into detrimental effects on mental and cardiometabolic health. This enables the development of novel monitoring and intervention strategies to track and reduce daily life stress and its health impact.